U.V Radiation Effect on Growth and Survival of Bacteria

Staphylococcus aureus

 

Sakshi Satyendranarayan Gupta

Department of Pharmaceutics, Gourishankar Institute of Pharmaceutical Education and Research, Limb, Satara. Dr. Babasaheb Ambedkar Technological University, Lonere, Maharashtra, India.

*Corresponding Author E-mail: sakshigupta2382000@gmail.com

 

ABSTRACT:

U.V radiation destroys the structure of DNA and other essential components of cell. In this study, U.V radiation effect on growth and survival of bacteria were studied. First spread plates of bacteria were prepared and exposed to U.V radiation with different time intervals (0, 2, 4, 6, and 8 minutes), then they are incubated at 37 o C for 24 hours. After incubation, number of colonies was calculated. If increase the U.V exposure time, the colony numbers were slowly decreased. In 8 minutes, treatment 104 colonies were observed. In the control treatment (0 minutes time exposed has 1146 colonies), 2 minutes (452 colonies), 4 minutes (278 colonies), 6 minutes (134 colonies) and 8 minutes (99 colonies) exposure reduce the colony number slowly. This bacterium has more resistant to U.V radiation.

 

KEYWORDS: Staphylococcus aureus, U.V radiation, Bacterial growth.

 

 

INTRODUCTION:

Many physical factors provide physiological stress to the living cells. The U. V radiation is one among them. The U.V radiation mainly damage DNA and causes genetic mutation1. The U.V radiation also alter the growth, virulence and physiology of microorganism. Our previous lab studies prove that U.V radiation mainly produces mutation in bacterial cells.2,3,4. The exposure of   U.V radiation is a major risk for most skin cancers in human beings. It is very harmful. However, it is used to treat some diseases such as rickets, psoriasis, eczema and jaundice5,6,7.

 

The bacteria Staphylococcus aureus causes food borne diseases. The food borne diseases are major concern worldwide8,9. Nearly around 250 different food borne diseases has been described and bacteria are the important causative agents. The Staphylococcus aureus is a leading cause of gastroenteritis resulting from consumption of contaminated food. The symptoms of food borne diseases are very widely, depending on the etiological agents. The common symptoms of food borne diseases are Diarrhea and Vomiting10,11,12,13. In this study, U.V radiation on the growth and survival of the bacteria Staphylococcus aureus was studied.

 

MATERIALS AND METHODS:

The bacterial pathogen was collected from patient’s samples in local hospital and it was cultured in Lab. The pathogen was confirmed by regular microbiological and biochemical tests. First bacterial spread plates were prepared. After that it was exposed to U.V radiation, at various time intervals of 0, 2, 4, 6, and 8 minutes and these plates were incubated at 37oC. After 24 hours the plates were taken, and colonies were counted with the help of colony counter. The experiments are carried out in triplicates.

 

RESULTS AND DISCUSSION:

U.V radiation produce mutation and sometimes, it produces and increase virulence due to genetic changes. The best mutant strains were used to prepare inactivated vaccines2,3,14. During increase the U.V radiation at certain limit, it produces mutation. These mutation leads to produce virulence. These virulence strains induce more immune response to host. The previous studies prove that six-minute U.V treated mutant strains produce good immune response4. In the present study, if increase the duration of U.V exposure time, the bacterial population slowly decreased. The eight-minute treatment has lesser number of colonies compared to other treatment. In the other hand, the best mutant strains only survive against U.V radiation. The non-mutant strains lose their survival. The bacteria have more resistant to U.V radiation.

 

Table-1 U.V radiation influence on Staphylococcus aureus bacterial colonies.

U.V Treatment Time(minutes)

Number of colonies

Control 0 minute

1146

2

452

4

278

6

134

8

99

 

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Received on 31.07.2020        Revised on 28.08.2020               

Accepted on 21.09.2020     ©Asian Pharma Press All Right Reserved

Asian J. Pharm. Ana. 2021; 11(1):27-28.

DOI: 10.5958/2231-5675.2021.00005.3